Name

Cord Factor, TDM

Synonyms

Isolated from Mycobacterium tuberculosis

Formulation

Lyophilised. Sterile.

CAS Number

61512-20-7

Purity

Single spot (TLC)

Endotoxin-free

0.002 EU/µg

Solubility

Soluble (5mg/ml) in chloroform:methanol:water (vol:vol) 90:9:1, hexane or isopropanol

Handling/Activity

Keep sterile. To stimulate mouse bone marrow-derived macrophages, Cord Factor was suspended at a concentration of 0.2mg/ml in hexane. Of the resulting solution 1 or 10µg/well were layered in 96-well tissue culture plates and the solvent completely evaporated. Control wells were layered with solvent without Cord Factor and also incubated at 37°C. To this layer of Cord Factor, bone marrow-derived macrophages were added in 100µl of medium and incubated at 37°C for 24 hours before activation e.g. TNF-α production was measured in the supernatant. In vivo pulmonary granuloma formation in mice can be induced by 10µg Cord Factor per mouse applied i.v. in a water/oil/water emulsion.