ODN 1668 (Type B) Endotoxin-free (sterile) Cat. No. #IAX-200-001

£375.81

Description Features Resources

Sequence	5’-tccatgacgttcctgatgct-3’ (lower case letters: phosphorothioate linkage: nuclease resistant)
Synonyms	CpG ODN TLR9 Agonist: B Type (species: rodent)
MW	6,383 g/mol
Formulation	Lyophilised. Sterile. 100µg size includes 1.5ml ddWater Endotoxin-free (sterile) (Cat. No.: IAX-900-002-LD15).1mg size includes 10ml ddWater Endotoxin-free (sterile) (Cat. No.: IAX-900-002-L010).
Endotoxin-free	Bacterial Endotoxin Test (kinetic turbidimetric LAL method) according to Ph. Eur. 9. Passed according to specification: Endotoxin-free: <0.002 EU/µg.
Sterility	Filter method: according to Ph. Eur. 9. Passed according to specification: • No growth in Thioglycolate medium at 30-35°C after 14 days. • No growth in Soybean Casein Digest Broth (TSB) at 20-25°C after 14 days.

Handling	Keep sterile. Reconstitution: Dissolve total vial content in sterile endotoxin-free water or PBS. Add 50% of solvent and let dissolve for 10mins. Add remaining 50% of the solvent and mix thoroughly. Moderate warming may aid dissolving.
Activity	Optimal concentration is dependent upon cell type, species, desired activation and analysis: in vitro 0.1-1µM (~0.6-6.0µg/ml); in vivo 50-250µg in rodent animal models. Negative control: GpC ODN 1720 (Cat. No.: IAX-200-200). As an alternative control without agonistic nor antagonistic activity consider CTRL2-ODN (Cat. No.: IAX-200-208).

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