Monophosphoryl Lipid A [MPLA] S. minnesota R595 (Re) TLRpure Sterile Cat. No. #IAX-100-002

£288.00

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Source

Monophosphoryl Lipid A [MPLA] derived from S. minnesota R595 (Re) TLRpureTM LPS

Concentration

1mg/ml (0.5mg/ml for 250µg size) stabilised in sterile, double-distilled water (ddWater) without any additives

TLRpureTM

No detectable TLR4 independent activity: standardised potent TLR4-specific agonist

Purity

Ultrapure. No detectable DNA, RNA and protein traces.

Purification Method

R-type (mutant/rough) LPS was isolated by a phenol-chloroform-petroleum-ether method. Semi-purified LPS was subjected to further re extraction cycles and ultracentrifugation steps, extensively electrodialysed before converted to its uniform sodium salt form to yield TLRpureTM LPS, from which MPLA was generated by acid hydrolysis.

Sterility

Filter method: according to Ph. Eur. 9.

Passed according to specification:

• No growth in Thioglycolate medium at 30-35°C after 14 days.

• No growth in Soybean Casein Digest Broth (TSB) at 20-25°C after 14 days.

Endotoxin Content

Bacterial Endotoxin Test (kinetic turbidimetric LAL method) according to Ph. Eur. 9.

Endotoxin Content: >1,000,000 [EU/ml].

Appearance

Colourless, clear to opaque aqueous solution

Handling

Keep sterile. Prepare working dilutions from pre-warmed (~40°C) MPLA stock solution just prior to use. Ready-made solution is cell culture-grade. Do not pre-dilute in buffer (e.g. PBS) as this will lead to precipitation of MPLA. To yield a 100µg/ml (1,000-100x) stock solution add 100µl of MPLA to 900µl endotoxin-free and sterile ddWater (Cat. No.: IAX-900-002) (not PBS) and mix well.

Activity

Optimal concentration is dependent upon cell type, species, desired activation and analysis:
0.1-1.0µg/ml in vitro and 5-15mg/kg in vivo in animal rodent models. Does not activate any TLR other than TLR4 as tested up to 1µg/ml in relevant cellular systems (mouse macrophages).


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Product Data Sheet
Safety Data Sheet

Brand Innaxon
Code IAX-100-002-M001

Product Data Sheets

 IAX-100-001 Lipid A from S. minnesota R595 (Re) TLRpureTM Sterile Solution
 IAX-100-002 MPLA from S. minnesota R595 (Re) TLRpureTM Sterile Solution
 IAX-100-003 MPLA from E coli R515 (Re) TLRpureTM Sterile Solution
 IAX-100-004 Lipid A from E. coli R515 (Re) TLRpureTM Sterlie Solution
 IAX-100-006 LPS from E. coli O8:K27 (S-form) TLRpureTM Sterile Solution
 IAX-100-007 LPS from E. coli R515 (Re) TLRpureTM Sterile Solution 
 IAX-100-008 LPS from S. minnesota R595 (Re) TLRpureTM Sterile Solution
 IAX-100-009 LPS (Universal) from S. abortus equi (S-form) TLRpureTM Sterile Solution
 IAX-100-009B LPS from S. abortus equi (S-form) Biotin TLRpureTM Sterile Solution
 IAX-100-010 LPS from E. coli EH100 (Ra) TLRpureTM Sterile Solution
 IAX-100-011 LPS from S. typhimurium (S-form) TLRpureTM Sterile Solution
 IAX-100-012 LPS from E. coli O111:B4 (S-form) TLRpureTM Sterile Solution
 IAX-100-013 LPS from E. coli O55:B5 (S-form) TLRpureTM Sterile Solution
 IAX-100-014 LPS from E. coli J5 (Rc) TLRpureTM Sterile Solution
 IAX-100-015 LPS from S. minnesota R345 (Rb) TLRpureTM Sterile Solution
 IAX-100-016 LPS from S. minnesota R60 (Ra) TLRpureTM Sterile Solution
 IAX-100-017 LPS from S. minnesota R5 (Rc) TLRpureTM Sterile Solution
 IAX-100-018 LPS from S. minnesota R7 (Rd1) TLRpureTM Sterile Solution
 IAX-100-019 LPS from S. enteritidis (S-form) TLRpureTM Sterile Solution
 IAX-100-020 LPS from S. minnesota (S-form) TLRpureTM Sterile Solution
 IAX-100-021 LPS from S. minnesota R3 (Rd2) TLRpureTM Sterile Solution

 IAX-200-001 ODN 1668 (Type B) Endotoxin-free (sterile)
 IAX-200-002 ODN 1826 (Type B) Endotoxin-free (sterile)
 IAX-200-003 ODN 1585 (Type A) Endotoxin-free (sterile)
 IAX-200-004 ODN M362 (Type C) Endotoxin-free (sterile)
​​​​​​​ IAX-200-005 ODN 2216 (Type A) Endotoxin-free (sterile)
​​​​​​​ IAX-200-006 ODN 2006 (Type B) Endotoxin-free (sterile)
​​​​​​​ IAX-200-007 ODN 2395 (Type C) Endotoxin-free (sterile)

​​​​​​​ IAX-200-050 iODN 2088 (Class I) Endotoxin-free (sterile)
​​​​​​​ IAX-200-051 iODN (ttaggg)4 (Class II) Endotoxin-free (sterile)
​​​​​​​ IAX-200-052 G-type iODN (Class I) Endotoxin-free (sterile)
​​​​​​​ IAX-200-053 Mini-iODN (Class I) Endotoxin-free (sterile)
​​​​​​​ IAX-200-054 Mega-iODN (Class I/ll) Endotoxin-free (sterile)
​​​​​​​ IAX-200-055 Duo-iODN (Class I) Endotoxin-free (sterile)
​​​​​​​ IAX-200-056 TLR7-iODN (inhibitory ODN) Endotoxin-free (sterile)

​​​​​​​ IAX-200-101 Trehalose 6,6’-dimycolate [Cord Factor] Endotoxin-free (sterile)

​​​​​​​ IAX-200-200 ODN 1720 (Control for ODN 1668) Endotoxin-free (sterile)
​​​​​​​ IAX-200-201 ODN 2138 (Control for ODN 1826) Endotoxin-free (sterile)
​​​​​​​ IAX-200-202 Neutral-ODN (Control for iODNs) Endotoxin-free (sterile)
​​​​​​​ IAX-200-203 ODN 2118 (Control for ODN 1585) Endotoxin-free (sterile)
​​​​​​​ IAX-200-204 ODN M383 (Control for ODN M362) Endotoxin-free (sterile)
​​​​​​​ IAX-200-205 ODN 2243 (Control for ODN 2216) Endotoxin-free (sterile)
​​​​​​​ IAX-200-206 ODN 2137 (Control for ODN 2006) Endotoxin-free (sterile)
​​​​​​​ IAX-200-208 CTRL2-ODN (Control for iODN and CpG-ODNs) Endotoxin-free (sterile)

​​​​​​​ IAX-400-002 Anti-TLR4 (Human) mAb (Blocking)
​​​​​​​ IAX-400-003 Anti-TLR4 (Human) mAb (2EII)
​​​​​​​ IAX-400-004 Anti-TLR4 (human) mAb (28H7)

​​​​​​​ IAX-600-001 IAXO-101
​​​​​​​ IAX-600-002 IAXO-102
​​​​​​​ ​​​​​​​IAX-600-003 IAXO-103
​​​​​​​ IAX-600-004 IAXO-201 (Control for IAXO-102)
​​​​​​​ IAX-600-005 IAXO-202 (Control for IAXO-101 and IAXO-103)

​​​​​​​ IAX-700-001 Argania Spinosa Kernel Oil (natural) (pure)

​​​​​​​ IAX-700-100 Lipodisq Control (sterile)
​​​​​​​ IAX-700-101 Nano-Curcumin Lipodisq (sterile)
​​​​​​​ IAX-700-102 Melatonin Lipodisq (sterile)
​​​​​​​ IAX-700-103 Metformin Lipodisq (sterile)
​​​​​​​ IAX-700-104 Oxyresveratrol Lipodisq (sterile)
​​​​​​​ IAX-700-105 Resveratrol Lipodisq (sterile)
​​​​​​​ IAX-700-106 Umifenovir Lipodisq (sterile)
​​​​​​​ IAX-700-107 Dexamethasone Lipodisq (sterile)
​​​​​​​ IAX-700-108 Ambroxol Lipodisq (sterile)
 
​​​​​​​ IAX-900-001 PBS Endotoxin-free (sterile)
​​​​​​​ IAX-900-002 ddWater Endotoxin-free (sterile)
​​​​​​​ ​​​​​​​IAX-900-003 Physiological Saline [NaCl 0.9%] Endotoxin-free (sterile)
​​​​​​​ IAX-900-004 PBS with EDTA Endotoxin-free (sterile)
​​​​​​​ IAX-900-005 TRIS with EDTA (TE Buffer) (100x)​​​​​​​
​​​​​​


Safety Data Sheets

​​​​​​​ IAX-100-001 Lipid A from S. minnesota R595 (Re) TLRpureTM Sterile Solution
​​​​​​​ IAX-100-002 MPLA from S. minnesota R595 (Re) TLRpureTM Sterile Solution
​​​​​​​ IAX-100-003 MPLA from E coli R515 (Re) TLRpureTM Sterile Solution
​​​​​​​ IAX-100-004 Lipid A from E. coli R515 (Re) TLRpureTM Sterlie Solution
​​​​​​​ IAX-100-006 LPS from E. coli O8:K27 (S-form) TLRpureTM Sterile Solution
​​​​​​​ IAX-100-007 LPS from E. coli R515 (Re) TLRpureTM TLRpureTM Sterile Solution
​​​​​​​ IAX-100-008 LPS from S. minnesota R595 (Re) TLRpureTM Sterile Solution
​​​​​​​ IAX-100-009 LPS (Universal) from S. abortus equi (S-form) TLRpureTM Sterile Solution
​​​​​​​ IAX-100-009B LPS from S. abortus equi (S-form) Biotin TLRpureTM Sterile Solution
​​​​​​​ IAX-100-010 LPS from E. coli EH100 (Ra) TLRpureTM TLRpureTM Sterile Solution
​​​​​​​ IAX-100-011 LPS from S. typhimurium (S-form) TLRpureTM Sterile Solution
​​​​​​​ IAX-100-012 LPS from E. coli O111:B4 (S-form) TLRpureTM Sterile Solution
​​​​​​​ IAX-100-013 LPS from E. coli O55:B5 (S-form) TLRpureTM Sterile Solution
​​​​​​​ IAX-100-014 LPS from E. coli J5 (Rc) TLRpureTM Sterile Solution
​​​​​​​ IAX-100-015 LPS from S. minnesota R345 (Rb) TLRpureTM Sterile Solution
​​​​​​​ IAX-100-016 LPS from S. minnesota R60 (Ra) TLRpureTM Sterile Solution
​​​​​​​ IAX-100-017 LPS from S. minnesota R5 (Rc) TLRpureTM Sterile Solution
​​​​​​​ IAX-100-018 LPS from S. minnesota R7 (Rd1) TLRpureTM Sterile Solution
​​​​​​​ ​​​​​​​IAX-100-019 LPS from S. enteritidis (S-form) TLRpureTM Sterile Solution
​​​​​​​ IAX-100-020 LPS from S. minnesota (S-form) TLRpureTM Sterile Solution
​​​​​​​ IAX-100-021 LPS from S. minnesota R3 (Rd2) TLRpureTM Sterile Solution

​​​​​​​ IAX-200-001 ODN 1668 (Type B) Endotoxin-free (sterile)
​​​​​​​ IAX-200-002 ODN 1826 (Type B) Endotoxin-free (sterile)
​​​​​​​ IAX-200-003 ODN 1585 (Type A) Endotoxin-free (sterile)
​​​​​​​ IAX-200-004 ODN M362 (Type C) Endotoxin-free (sterile)
​​​​​​​ IAX-200-005 ODN 2216 (Type A) Endotoxin-free (sterile)
​​​​​​​ IAX-200-006 ODN 2006 (Type B) Endotoxin-free (sterile)
​​​​​​​ IAX-200-007 ODN 2395 (Type C) Endotoxin-free (sterile)

​​​​​​​​​​​​​​ IAX-200-050 iODN 2088 (Class I) Endotoxin-free (sterile)
​​​​​​​ IAX-200-051 iODN (ttaggg)4 (Class II) Endotoxin-free (sterile)
​​​​​​​ IAX-200-052 G-type iODN (Class I) Endotoxin-free (sterile)
​​​​​​​ IAX-200-053 Mini-iODN (Class I) Endotoxin-free (sterile)
​​​​​​​ IAX-200-054 Mega-iODN (Class I/ll) Endotoxin-free (sterile)
​​​​​​​ IAX-200-055 Duo-iODN (Class I) Endotoxin-free (sterile)
​​​​​​​ IAX-200-056 TLR7-iODN (inhibitory ODN) Endotoxin-free (sterile)

​​​​​​​ IAX-200-101 Trehalose 6,6’-dimycolate [Cord Factor] Endotoxin-free (sterile)

​​​​​​​ IAX-200-200 ODN 1720 (Control for ODN 1668) Endotoxin-free (sterile)
​​​​​​​ IAX-200-201 ODN 2138 (Control for ODN 1826) Endotoxin-free (sterile)
​​​​​​​ IAX-200-202 Neutral-ODN (Control for iODNs) Endotoxin-free (sterile)
​​​​​​​ IAX-200-203 ODN 2118 (Control for ODN 1585) Endotoxin-free (sterile)
​​​​​​​ IAX-200-204 ODN M383 (Control for ODN M362) Endotoxin-free (sterile)
​​​​​​​ IAX-200-205 ODN 2243 (Control for ODN 2216) Endotoxin-free (sterile)
​​​​​​​ IAX-200-206 ODN 2137 (Control for ODN 2006) Endotoxin-free (sterile)
​​​​​​​ IAX-200-208 CTRL2-ODN (Control for iODN and CpG-ODNs) Endotoxin-free (sterile)

​​​​​​​ IAX-400-002 Anti-TLR4 (Human) mAb (Blocking)
​​​​​​​ IAX-400-003 Anti-TLR4 (Human) mAb (2EII)
​​​​​​​ IAX-400-004 Anti-TLR4 (human) mAb (28H7)

​​​​​​​ IAX-600-001 IAXO-101
​​​​​​​ IAX-600-002 IAXO-102
​​​​​​​ IAX-600-003 IAXO-103
​​​​​​​ IAX-600-004 IAXO-201 (Control for IAXO-102)
​​​​​​​ IAX-600-005 IAXO-202 (Control for IAXO-101 and IAXO-103)

​​​​​​​ IAX-700-001 Argania Spinosa Kernel Oil (natural) (pure)

​​​​​​​ ​​​​​​​IAX-700-100 Lipodisq Control (sterile)
​​​​​​​ IAX-700-101 Nano-Curcumin Lipodisq (sterile)
​​​​​​​ IAX-700-102 Melatonin Lipodisq (sterile)
​​​​​​​ IAX-700-103 Metformin Lipodisq (sterile)
​​​​​​​ IAX-700-104 Oxyresveratrol Lipodisq (sterile)
​​​​​​​ IAX-700-105 Resveratrol Lipodisq (sterile)
​​​​​​​ ​​​​​​​IAX-700-106 Umifenovir Lipodisq (sterile)
​​​​​​​ ​​​​​​​IAX-700-107 Dexamethasone Lipodisq (sterile)
​​​​​​​ IAX-700-108 Ambroxol Lipodisq (sterile)

​​​​​​​ IAX-900-001 PBS Endotoxin-free (sterile)
​​​​​​​ IAX-900-002 ddWater Endotoxin-free (sterile)
​​​​​​​ IAX-900-003 Physiological Saline [NaCl 0.9%] Endotoxin-free (sterile)
​​​​​​​ IAX-900-004 PBS with EDTA Endotoxin-free (sterile)
​​​​​​​ IAX-900-005 TRIS with EDTA (TE Buffer) (100x)​​​​​​​

​​​​​​​

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