Monophosphoryl Lipid A [MPLA] E. coli R515 (Re) TLRpure Sterile Cat. No. #IAX-100-003
Source |
Monophosphoryl Lipid A [MPLA] derived from E. coli R515 (Re) LPS |
Concentration |
1mg/ml (0.5mg/ml for 250µg size) stabilised in sterile, double-distilled water (ddWater), without any additives |
TLRpureTM |
No detectable TLR4 independent activity: standardised potent TLR4-specific agonist |
Purity |
Ultrapure. No detectable DNA, RNA and protein traces. |
Purification Method |
R-type (mutant/rough) LPS was isolated by a phenol-chloroform-petroleum-ether method. Semi-purified LPS was subjected to further re-extraction cycles and ultracentrifugation steps, extensively electrodialysed to yield LPS, from which MPLA was generated by acid hydrolysis. |
Sterility |
Filter method: according to Ph. Eur. 9. Passed according to specification: • No growth in Thioglycolate medium at 30-35°C after 14 days. • No growth in Soybean Casein Digest Broth (TSB) at 20-25°C after 14 days. |
Endotoxin Content |
Bacterial Endotoxin Test (kinetic turbidimetric LAL method) according to Ph. Eur. 9. Endotoxin Content: >1,000,000 [EU/ml]. |
Appearance |
Colourless, clear to opaque aqueous solution |
Handling |
Keep sterile. Prepare working dilutions from pre-warmed (~40°C) MPLA stock solution just prior to use. Ready-to-use, sterile stock solution is cell culture-grade. No solubilisation required. Do not pre-dilute in buffer (e.g. PBS) as this will lead to precipitation of MPLA. To yield a 100µg/ml (1,000-100x) stock solution add 100µl of MPLA to 900µl endotoxin-free and sterile ddWater (Cat. No.: IAX-900-002) (not PBS) and mix well. |
Activity |
Optimal concentration is dependent upon cell type, species, desired activation and analysis: |
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Brand | Innaxon |
Code | IAX-100-003-M001 |
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