Monophosphoryl Lipid A [MPLA] (bio-synthetic) from E. coli R515 (Re) TLRpure Sterile Solution

£201.82

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Source
Monophosphoryl Lipid A [MPLA] derived from E. coli R515 (Re) TLRpure™ LPS (bio-synthetic).


TLRpure™
No detectable TLR4 independent activity: standardised potent TLR4-specific agonist.


Purity
≥99.9%. No detectable DNA, RNA and protein traces.


Purification Method
R-form (mutant/rough) LPS was isolated by phenol-chloroform-petroleum-ether method. Semi-purified LPS was subjected to further re-extraction cycles and ultracentrifugation steps, extensively electrodialysed before converted to its uniform sodium salt form to yield TLRpure™ LPS, from which MPLA was generated by acid hydrolysis.


Concentration  
1mg/ml (0.5mg/ml for 250μg size) stabilised in sterile, double-distilled water (ddWater), without any additives.


Biological Activity
Optimal concentration is dependent upon cell type, species, desired activation and analysis: 0.1-1.0μg/ml. Does not activate any TLR other than TLR4 as tested up to 50μg/ml in relevant cellular systems (macrophages).


Shipping            
AMBIENT


Storage
+4°C


Handling Advice
Do not freeze. Prepare diluted Lipid A working solutions in water just prior to use, keep sterile. Do not pre-dilute in buffer (e.g. PBS) as this will lead to precipitation of MPLA. To yield a 100μg/ml (1,000-100x) stock solution add 100μl of Lipid A to 900μl endotoxin-free sterile  ddWater (Cat. No.: IAX-900-002) (not PBS) and mix well. Ready-made solution is cell culture-grade.


Stability
Stable for at least 2 years after receipt when stored at +4°C.


 

We ensure that our products fulfill the following:

  • Qualified Purity & Activity  
  • High potency TLR4-specific Ligand
  • Ultrapure lipopolysaccharides (LPS) (no detectable activity induced by protein, RNA and DNA)
  • Tested on TLRA KO murine macrophages
  • Standardised Aqueous Sterile Solution
  • No purification or hazardous and variable solubilisation required
  • Excellent lot-to-lot consistency due to large batch production
  • Bio-synthetic MPLA with higher LAL gel clotting and biological potency compared to synthetic (artifical) MPLA
Brand Innaxon
Code IAX-100-003-M001
Product Data Sheets
 

 Lipid A from E. coli R515 (Re) TLRpureTM Sterile Solution
 Lipid A from S. minnesota R595 (Re) TLRpureTM Sterile Solution
 MPLA from E coli R515 (Re) TLRpureTM Sterile Solution
 
MPLA from S. minnesota R595 (Re) TLRpureTM Sterile Solution

 LPS from E. coli O8:K27 (S-form) TLRpureTM Sterile Solution
 LPS from E. coli EH100 (Ra) TLRpureTM Sterile Solution
 LPS from E. coli O111:B4 (S-form) TLRpureTM Sterile Solution
 LPS from E. coli O55:B5 (S-form) TLRpureTM Sterile Solution
 LPS from E. coli J5 (Rc) TLRpureTM Sterile Solution

TLR4 Agonist (E. coli) Explorer Set I TLRpureTM
 TLR4 Agonist (E. coli) Explorer Set II TLRpureTM


 LPS (Universal) from S. abortus equi (S-form) TLRpureTM Sterile Solution
 LPS from S. abortus equi (S-form) Biotin TLRpureTM Sterile Solution
 LPS from S. minnesota R595 (Re) TLRpureTM Sterile Solution
 LPS from S. enteritidis (S-form) TLRpureTM Sterile Solution
 LPS from S. minnesota R345 (Rb) TLRpureTM Sterile Solution
 LPS from S. minnesota R60 (Ra) TLRpureTM Sterile Solution
 LPS from S. minnesota R7 (Rd1) TLRpureTM Sterile Solution
 LPS from S. minnesota (S-form) TLRpureTM Sterile Solution
 LPS from S. typhimurium (S-form) TLRpureTM Sterile Solution

 TLR4 Agonist (Salmonella) Explorer Set I TLRpureTM
 TLR4 Agonist (Salmonella) Explorer Set II TLRpureTM


 iODN 2088 (Class I) Endotoxin-free (sterile)
ODN iODN (ttaggg)4 (Class II) Endotoxin-free (sterile)
G-type iODN (Class I) Endotoxin-free (sterile)
Duo-iODN (Class I) Endotoxin-free (sterile)
Mini-iODN (Class I) Endotoxin-free (sterile)

Mega-iODN (Class I/ll) Endotoxin-free (sterile)

 iODN Explorer Set I Endotoxin-free (sterile)

Neutral-ODN (Control for iODNs) Endotoxin-free (sterile)

 ODN 2216 (Type A) Endotoxin-free (sterile)
ODN 2006 (Type B) Endotoxin-free (sterile)
 ODN 2395 (Type C) Endotoxin-free (sterile)
ODN 1585 (Type A) Endotoxin-free (sterile)
ODN 1668 (Type B) Endotoxin-free (sterile)
 ODN 1826 (Type B) Endotoxin-free (sterile)
ODN M362 (Type C) Endotoxin-free (sterile)


TLR9 Agonist (human) Explorer Set Endotoxin-free (sterile)
TLR9 Agonist (mouse) Explorer Set Endotoxin-free (sterile)

ODN 2243 (Control for ODN 2216) Endotoxin-free (sterile)
ODN 2137 (Control for ODN 2006) Endotoxin-free (sterile)
 ODN 2118 (Control for ODN 1585) Endotoxin-free (sterile)
ODN 1720 (Control for ODN 1668) Endotoxin-free (sterile)
ODN 1982 (Control for ODN 1826) Endotoxin-free (sterile)
ODN M383 (Control for ODN M362) Endotoxin-free (sterile)

 IAXO-101
 IAXO-102
 IAXO-103
 IAXO-201
 IAXO-202


PBS Endotoxin-free (sterile)
Physiological Saline [Sodium Chloride 0.9%] Endotoxin-free (sterile)
 ddWater Endotoxin-free (sterile)


 Argania Spinosa Kernel Oil (natural) (pure)

 Trehalose 6,6’-dimycolate [Cord Factor] Endotoxin-free (sterile)

 

Need additional information?
Please get in touch via email: info@innaxon.com