Mega-iODN (inhibitory ODN) Endotoxin-free (sterile) Cat. No. #IAX-200-054

£98.00

Description Features Resources

Sequence	5’-cctcaatagggtgagggg-3’ (lower case letters: phosphorothioate linkage: nuclease resistant)
Synonyms	Inhibitory ODN (iODN): Class I/II
MW	5,894 g/mol
Formulation	Lyophilised. Sterile. 100µg size includes 1.5ml ddWater Endotoxin-free (sterile) (Cat. No.: IAX-900-002-LD15). 1mg size includes 10ml ddWater Endotoxin-free (sterile) (Cat. No.: IAX-900-002-L010).
Endotoxin-free	Bacterial Endotoxin Test (kinetic turbidimetric LAL method) according to Ph. Eur. 9. Passed according to specification: Endotoxin-free: <0.002 EU/µg.
Sterility	Filter method: according to Ph. Eur. 9. Passed according to specification: • No growth in Thioglycolate medium at 30-35°C after 14 days. • No growth in Soybean Casein Digest Broth (TSB) at 20-25°C after 14 days.

Handling	Keep sterile. Reconstitution: Dissolve total vial content in sterile endotoxin-free water or PBS. Add 50% of solvent and let dissolve for 10min. Add remaining 50% of the solvent and mix thoroughly. Moderate warming may aid dissolving.
Activity	Particularly potent sequence of an inhibitory ODN for in vivo use in rodents (50-150ug per injection): based on class I (ODN H154) and class II (ODN A151) sequences to combine inhibitory activity on TLRs and STAT signalling. Negative control: CTRL2-ODN (Control for iODN and CpG-ODNs) (Cat. No.: IAX-200-208).

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