LPS (Universal) S. abortus equi (S-form) TLRpure Sterile Aqueous Solution Cat. No. #IAX-100-009

£132.26

Description Features Resources

Source	Lipopolysaccharide (LPS) from Salmonella abortus equi, S-type (smooth/wild-type) LPS
Concentration	1mg/ml stabilised in sterile, double-distilled water (ddWater), without any additives
TLRpure^TM	No detectable TLR4 independent activity as determined by a mouse macrophage cell culture cytokine secretion assay using TLR4 deficient versus wild-type cells: standardised potent TLR4-specific agonist
Purity	Ultrapure. No detectable DNA, RNA and protein traces.
Purification Method	S-type LPS was isolated by a modified phenol-chloroform-petroleum ether method. Semi-purified LPS was subjected to further re-extraction cycles and ultracentrifugation steps, extensively electrodialysed to yield TLRpure^TM LPS.
Sterility	Filter method: certified according to Ph. Eur. 9. Passed according to specification: • No growth in Thioglycolate medium at 30-35°C after 14 days. • No growth in Soybean Casein Digest Broth (TSB) at 20-25°C after 14 days.
Endotoxin Content	Bacterial Endotoxin Test (kinetic turbidimetric LAL method) certified according to Ph. Eur. 9. Endotoxin Content: >5,000,000 [EU/ml].

Appearance	Colourless, clear, aqueous solution
Handling	Keep sterile. Prepare aliquots or working dilutions from pre-warmed (~40°C) LPS stock solution just prior to use. Ready-to-use, sterile stock solution is cell culture-grade. No solubilisation required. To yield a 100µg/ml (100x) stock solution, add 100µl of LPS to 900µl endotoxin-free and sterile ddWater (Cat. No.: IAX-900-002), or 0.9% NaCl Solution (Cat. No.: IAX-900-003) or PBS (Cat. No.: IAX-900-001) and mix well.
Activity	Optimal concentration is dependent upon cell type, species, desired activation and analysis: 0.01-1.0µg/ml in vitro and 5-15mg/kg in vivo in animal rodent models. Does not activate any TLR other than TLR4 as tested up to 10µg/ml in relevant cellular systems (mouse macrophages).

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