LPS from E. coli O111:B4 (S-form) TLRpure Sterile Solution

£111.94

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Source
Lipopolysaccharide (LPS) from E. coli O111:B4, S-type (smooth/wild-type) LPS.


TLRpure™
No detectable TLR4 independent activity: standardised potent TLR4-specific agonist.


Purity
≥99.9%. No detectable DNA, RNA and protein traces.


Purification Method
S-type LPS was isolated by the hot phenol-water method followed by a modified phenol-chloroform-petroleumether method. Semi-purified LPS was subjected to further re-extraction cycles and ultracentrifugation steps, extensively electrodialysed before converted to its uniform sodium salt form to yield TLRpure™ LPS.


Concentration  
1mg/ml stabilised in sterile, double-distilled water (ddWater), without any additives.


Biological Activity
Optimal concentration is dependent upon cell type, species, desired activation and analysis: 0.01-1.0μg/ml. Does not activate any TLR other than TLR4 as tested up to 50μg/ml in relevant cellular systems (macrophages).


Shipping            
AMBIENT


Storage
+4°C


Handling Advice
Do not freeze. Prepare diluted LPS working solutions just prior to use, keep sterile. Ready-made solution is cell culture-grade. To yield a 100μg/ml (1,000-100x) stock solution add 100μl of LPS to 900μl endotoxin-free and sterile ddWater (Cat. No.: IAX-900-002), 0.9% NaCl Solution (Cat. No.: IAX-900-003) or PBS (Cat. No.: IAX-900-001) and mix well.


Stability
Stable for at least 2 years after receipt when stored at +4°C.


 

We ensure that our products fulfill the following:

  • Qualified Purity & Activity  
  • High potency TLR4-specific Ligand
  • Ultrapure (no detectable protein, RNA and DNA)
  • Tested on TLR4 KO murine macrophages
  • Standardised Aqueous Sterile Solution
  • No purification or hazardous solubilisation
  • Excellent lot-to-lot consistency
Brand Innaxon
Code IAX-100-012-M001
Product Data Sheets


 Lipid A from E. coli R515 (Re) TLRpureTM Sterlie Solution Product Data Sheet
 Lipid A from S. minnesota R595 (Re) TLRpureTM Sterile Solution Product Data Sheet
 MPLA from E coli R515 (Re) TLRpureTM Sterile Solution Product Data Sheet
 
MPLA from S. minnesota R595 (Re) TLRpureTM Sterile Solution Product Data Sheet

 LPS from E. coli O8:K27 (S-form) TLRpureTM Sterile Solution Product Data Sheet
 LPS from E. coli EH100 (Ra) TLRpureTM Sterile Solution Product Data Sheet
 LPS from E. coli O111:B4 (S-form) TLRpureTM Sterile Solution Product Data Sheet
 LPS from E. coli O55:B5 (S-form) TLRpureTM Sterile Solution Product Data Sheet
 LPS from E. coli J5 (Rc) TLRpureTM Sterile Solution Product Data Sheet

TLR4 Agonist (E. coli) Explorer Set I TLRpureTM Product Data Sheet
 TLR4 Agonist (E. coli) Explorer Set II TLRpureTM Product Data Sheet


 LPS (Universal) from S. abortus equi (S-form) TLRpureTM Sterile Solution Product Data Sheet
 LPS from S. abortus equi (S-form) Biotin TLRpureTM Sterile Solution Product Data Sheet
 LPS from S. minnesota R595 (Re) TLRpureTM Sterile Solution Product Data Sheet
 LPS from S. enteritidis (S-form) TLRpureTM Sterile Solution Product Data Sheet
 LPS from S. minnesota R345 (Rb) TLRpureTM Sterile Solution Product Data Sheet
 LPS from S. minnesota R60 (Ra) TLRpureTM Sterile Solution Product Data Sheet
 LPS from S. minnesota R7 (Rd1) TLRpureTM Sterile Solution Product Data Sheet
 LPS from S. minnesota (S-form) TLRpureTM Sterile Solution Product Data Sheet
 LPS from S. typhimurium (S-form) TLRpureTM Sterile Solution Product Data Sheet

 TLR4 Agonist (Salmonella) Explorer Set I TLRpureTM Product Data Sheet
 TLR4 Agonist (Salmonella) Explorer Set II TLRpureTM Product Data Sheet


 iODN 2088 (Class I) Endotoxin-free (sterile) Product Data Sheet
iODN (ttaggg)4 (Class II) Endotoxin-free (sterile) Product Data Sheet
G-type iODN (Class I) Endotoxin-free (sterile) Product Data Sheet
Duo-iODN (Class I) Endotoxin-free (sterile) Product Data Sheet
Mini-iODN (Class I) Endotoxin-free (sterile) Product Data Sheet

Mega-iODN (Class I/ll) Endotoxin-free (sterile) Product Data Sheet

 iODN Explorer Set I Endotoxin-free (sterile) Product Data Sheet

Neutral-ODN (Control for iODNs) Endotoxin-free (sterile) Product Data Sheet

 ODN 2216 (Type A) Endotoxin-free (sterile) Product Data Sheet
ODN 2006 (Type B) Endotoxin-free (sterile) Product Data Sheet
 ODN 2395 (Type C) Endotoxin-free (sterile) Product Data Sheet
ODN 1585 (Type A) Endotoxin-free (sterile) Product Data Sheet
ODN 1668 (Type B) Endotoxin-free (sterile) Product Data Sheet
 ODN 1826 (Type B) Endotoxin-free (sterile) Product Data Sheet
ODN M362 (Type C) Endotoxin-free (sterile) Product Data Sheet


TLR9 Agonist (human) Explorer Set Endotoxin-free (sterile) Product Data Sheet
TLR9 Agonist (mouse) Explorer Set Endotoxin-free (sterile) Product Data Sheet

ODN 2243 (Control for ODN 2216) Endotoxin-free (sterile) Product Data Sheet
ODN 2137 (Control for ODN 2006) Endotoxin-free (sterile) Product Data Sheet
 ODN 2118 (Control for ODN 1585) Endotoxin-free (sterile) Product Data Sheet
ODN 1720 (Control for ODN 1668) Endotoxin-free (sterile) Product Data Sheet
ODN 1982 (Control for ODN 1826) Endotoxin-free (sterile) Product Data Sheet
ODN M383 (Control for ODN M362) Endotoxin-free (sterile) Product Data Sheet

 IAXO-101 Product Data Sheet
 IAXO-102 Product Data Sheet
 IAXO-103 Product Data Sheet


PBS Endotoxin-free (sterile) Product Data Sheet
Physiological Saline [Sodium Chloride 0.9%] Endotoxin-free (sterile) Product Data Sheet
 ddWater Endotoxin-free (sterile) Product Data Sheet


 Argania Spinosa Kernel Oil (natural) (pure) Product Data Sheet

 Trehalose 6,6’-dimycolate [Cord Factor] Endotoxin-free (sterile) Product Data Sheet

 

Material Safety Data Sheets (MSDS)
 

 Lipid A from E. coli R515 (Re) TLRpureTM Sterlie Solution MSDS
 Lipid A from S. minnesota R595 (Re) TLRpureTM Sterile Solution MSDS
 MPLA from E coli R515 (Re) TLRpureTM Sterile Solution MSDS
 
MPLA from S. minnesota R595 (Re) TLRpureTM Sterile Solution MSDS

 LPS from E. coli O8:K27 (S-form) TLRpureTM Sterile Solution MSDS
 LPS from E. coli EH100 (Ra) TLRpureTM Sterile Solution MSDS
 LPS from E. coli O111:B4 (S-form) TLRpureTM Sterile Solution MSDS
 LPS from E. coli O55:B5 (S-form) TLRpureTM Sterile Solution MSDS
 LPS from E. coli J5 (Rc) TLRpureTM Sterile Solution MSDS


TLR4 Agonist (E. coli) Explorer Set I TLRpureTM MSDS
 TLR4 Agonist (E. coli) Explorer Set II TLRpureTM MSDS


 LPS (Universal) from S. abortus equi (S-form) TLRpureTM Sterile Solution MSDS
 LPS from S. abortus equi (S-form) Biotin TLRpureTM Sterile Solution MSDS
 LPS from S. minnesota R595 (Re) TLRpureTM Sterile Solution MSDS
 LPS from S. enteritidis (S-form) TLRpureTM Sterile Solution MSDS
 LPS from S. minnesota R345 (Rb) TLRpureTM Sterile Solution MSDS
 LPS from S. minnesota R60 (Ra) TLRpureTM Sterile Solution MSDS
 LPS from S. minnesota R7 (Rd1) TLRpureTM Sterile Solution MSDS
 LPS from S. minnesota (S-form) TLRpureTM Sterile Solution MSDS
 LPS from S. typhimurium (S-form) TLRpureTM Sterile Solution MSDS

 TLR4 Agonist (Salmonella) Explorer Set I TLRpureTM MSDS
 TLR4 Agonist (Salmonella) Explorer Set II TLRpureTM MSDS


 iODN 2088 (Class I) Endotoxin-free (sterile) MSDS
iODN (ttaggg)4 (Class II) Endotoxin-free (sterile) MSDS
G-type iODN (Class I) Endotoxin-free (sterile) MSDS
Duo-iODN (Class I) Endotoxin-free (sterile) MSDS
Mini-iODN (Class I) Endotoxin-free (sterile) MSDS

Mega-iODN (Class I/ll) Endotoxin-free (sterile) MSDS

 iODN Explorer Set I Endotoxin-free (sterile) MSDS

Neutral-ODN (Control for iODNs) Endotoxin-free (sterile) MSDS

 ODN 2216 (Type A) Endotoxin-free (sterile) MSDS
ODN 2006 (Type B) Endotoxin-free (sterile) MSDS
 ODN 2395 (Type C) Endotoxin-free (sterile) MSDS
ODN 1585 (Type A) Endotoxin-free (sterile) MSDS
ODN 1668 (Type B) Endotoxin-free (sterile) MSDS
 ODN 1826 (Type B) Endotoxin-free (sterile) MSDS
ODN M362 (Type C) Endotoxin-free (sterile) MSDS



TLR9 Agonist (human) Explorer Set Endotoxin-free (sterile) MSDS
TLR9 Agonist (mouse) Explorer Set Endotoxin-free (sterile) MSDS

ODN 2243 (Control for ODN 2216) Endotoxin-free (sterile) MSDS
ODN 2137 (Control for ODN 2006) Endotoxin-free (sterile) MSDS
 ODN 2118 (Control for ODN 1585) Endotoxin-free (sterile) MSDS
ODN 1720 (Control for ODN 1668) Endotoxin-free (sterile) MSDS
ODN 1982 (Control for ODN 1826) Endotoxin-free (sterile) MSDS
ODN M383 (Control for ODN M362) Endotoxin-free (sterile) MSDS


IAXO-101 MSDS
IAXO-102 MSDS
IAXO-103 MSDS


PBS Endotoxin-free (sterile) MSDS
Physiological Saline [Sodium Chloride 0.9%] Endotoxin-free (sterile) MSDS
ddWater Endotoxin-free (sterile) MSDS


Argania Spinosa Kernel Oil (natural) (pure) MSDS

Trehalose 6,6’-dimycolate [Cord Factor] Endotoxin-free (sterile) MSDS
 



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Please get in touch via email: info@innaxon.com