LPS E. coli EH100 (Ra) TLRpure Sterile Aqueous Solution Cat. No. #IAX-100-010
Source |
Lipopolysaccharide (LPS) from E. coli strain EH100 (Ra), R-type (rough/mutant) LPS |
Concentration |
1mg/ml stabilised in sterile, double-distilled water (ddWater), without any additives |
TLRpureTM |
No detectable TLR4 independent activity as determined by a mouse macrophage cell culture cytokine secretion assay using TLR4 deficient versus wild-type cells: standardised potent TLR4-specific agonist |
Purity |
Ultrapure. No detectable DNA, RNA and protein traces. |
Purification Method |
R-type (mutant/rough) LPS was isolated by phenol-chloroform-petroleum-ether method. Semi-purified LPS was subjected to further re-extraction cycles and ultracentrifugation steps, extensively electrodialysed to yield TLRpureTM LPS. |
Sterility |
Filter method: certified according to Ph. Eur. 9. Passed according to specification: • No growth in Thioglycolate medium at 30-35°C after 14 days. • No growth in Soybean Casein Digest Broth (TSB) at 20-25°C after 14 days. |
Endotoxin Content |
Bacterial Endotoxin Test (kinetic turbidimetric LAL method) certified according to Ph. Eur. 9. Endotoxin Content: >10,000,000 [EU/ml]. |
Appearance |
Colourless, clear, aqueous solution |
Handling |
Keep sterile. Prepare working dilutions from pre-warmed (~40°C) LPS stock solution just prior to use. Ready-to-use, sterile stock solution is cell culture-grade. No solubilisation required. To yield a 100µg/ml (1,000-100x) stock solution add 100µl of LPS to 900µl endotoxin-free and sterile ddWater (Cat. No.: IAX-900-002) or 0.9% NaCl Solution (Cat. No.: IAX-900-003) and mix well. |
Activity |
Optimal concentration is dependent upon cell type, species, desired activation and analysis: |
Download Product Resources
Brand | Innaxon |
Code | IAX-100-010-M001 |
Product Data Sheets
IAX-100-001 Lipid A from S. minnesota R595 (Re) TLRpureTM Sterile Solution
IAX-100-002 MPLA from S. minnesota R595 (Re) TLRpureTM Sterile Solution
IAX-100-003 MPLA from E coli R515 (Re) TLRpureTM Sterile Solution
IAX-100-004 Lipid A from E. coli R515 (Re) TLRpureTM Sterlie Solution
IAX-100-006 LPS from E. coli O8:K27 (S-form) TLRpureTM Sterile Solution
IAX-100-007 LPS from E. coli R515 (Re) TLRpureTM Sterile Solution
IAX-100-008 LPS from S. minnesota R595 (Re) TLRpureTM Sterile Solution
IAX-100-009 LPS (Universal) from S. abortus equi (S-form) TLRpureTM Sterile Solution
IAX-100-009B LPS from S. abortus equi (S-form) Biotin TLRpureTM Sterile Solution
IAX-100-010 LPS from E. coli EH100 (Ra) TLRpureTM Sterile Solution
IAX-100-011 LPS from S. typhimurium (S-form) TLRpureTM Sterile Solution
IAX-100-012 LPS from E. coli O111:B4 (S-form) TLRpureTM Sterile Solution
IAX-100-013 LPS from E. coli O55:B5 (S-form) TLRpureTM Sterile Solution
IAX-100-014 LPS from E. coli J5 (Rc) TLRpureTM Sterile Solution
IAX-100-015 LPS from S. minnesota R345 (Rb) TLRpureTM Sterile Solution
IAX-100-016 LPS from S. minnesota R60 (Ra) TLRpureTM Sterile Solution
IAX-100-017 LPS from S. minnesota R5 (Rc) TLRpureTM Sterile Solution
IAX-100-018 LPS from S. minnesota R7 (Rd1) TLRpureTM Sterile Solution
IAX-100-019 LPS from S. enteritidis (S-form) TLRpureTM Sterile Solution
IAX-100-020 LPS from S. minnesota (S-form) TLRpureTM Sterile Solution
IAX-100-021 LPS from S. minnesota R3 (Rd2) TLRpureTM Sterile Solution
IAX-200-001 ODN 1668 (Type B) Endotoxin-free (sterile)
IAX-200-002 ODN 1826 (Type B) Endotoxin-free (sterile)
IAX-200-003 ODN 1585 (Type A) Endotoxin-free (sterile)
IAX-200-004 ODN M362 (Type C) Endotoxin-free (sterile)
IAX-200-005 ODN 2216 (Type A) Endotoxin-free (sterile)
IAX-200-006 ODN 2006 (Type B) Endotoxin-free (sterile)
IAX-200-007 ODN 2395 (Type C) Endotoxin-free (sterile)
IAX-200-050 iODN 2088 (Class I) Endotoxin-free (sterile)
IAX-200-051 iODN (ttaggg)4 (Class II) Endotoxin-free (sterile)
IAX-200-052 G-type iODN (Class I) Endotoxin-free (sterile)
IAX-200-053 Mini-iODN (Class I) Endotoxin-free (sterile)
IAX-200-054 Mega-iODN (Class I/ll) Endotoxin-free (sterile)
IAX-200-055 Duo-iODN (Class I) Endotoxin-free (sterile)
IAX-200-056 TLR7-iODN (inhibitory ODN) Endotoxin-free (sterile)
IAX-200-101 Trehalose 6,6’-dimycolate [Cord Factor] Endotoxin-free (sterile)
IAX-200-200 ODN 1720 (Control for ODN 1668) Endotoxin-free (sterile)
IAX-200-201 ODN 2138 (Control for ODN 1826) Endotoxin-free (sterile)
IAX-200-202 Neutral-ODN (Control for iODNs) Endotoxin-free (sterile)
IAX-200-203 ODN 2118 (Control for ODN 1585) Endotoxin-free (sterile)
IAX-200-204 ODN M383 (Control for ODN M362) Endotoxin-free (sterile)
IAX-200-205 ODN 2243 (Control for ODN 2216) Endotoxin-free (sterile)
IAX-200-206 ODN 2137 (Control for ODN 2006) Endotoxin-free (sterile)
IAX-200-208 CTRL2-ODN (Control for iODN and CpG-ODNs) Endotoxin-free (sterile)
IAX-400-002 Anti-TLR4 (Human) mAb (Blocking)
IAX-400-003 Anti-TLR4 (Human) mAb (2EII)
IAX-400-004 Anti-TLR4 (human) mAb (28H7)
IAX-600-001 IAXO-101
IAX-600-002 IAXO-102
IAX-600-003 IAXO-103
IAX-600-004 IAXO-201 (Control for IAXO-102)
IAX-600-005 IAXO-202 (Control for IAXO-101 and IAXO-103)
IAX-700-001 Argania Spinosa Kernel Oil (natural) (pure)
IAX-700-100 Lipodisq Control (sterile)
IAX-700-101 Nano-Curcumin Lipodisq (sterile)
IAX-700-102 Melatonin Lipodisq (sterile)
IAX-700-103 Metformin Lipodisq (sterile)
IAX-700-104 Oxyresveratrol Lipodisq (sterile)
IAX-700-105 Resveratrol Lipodisq (sterile)
IAX-700-106 Umifenovir Lipodisq (sterile)
IAX-700-107 Dexamethasone Lipodisq (sterile)
IAX-700-108 Ambroxol Lipodisq (sterile)
IAX-900-001 PBS Endotoxin-free (sterile)
IAX-900-002 ddWater Endotoxin-free (sterile)
IAX-900-003 Physiological Saline [NaCl 0.9%] Endotoxin-free (sterile)
IAX-900-004 PBS with EDTA Endotoxin-free (sterile)
IAX-900-005 TRIS with EDTA (TE Buffer) (100x)
Safety Data Sheets
IAX-100-001 Lipid A from S. minnesota R595 (Re) TLRpureTM Sterile Solution
IAX-100-002 MPLA from S. minnesota R595 (Re) TLRpureTM Sterile Solution
IAX-100-003 MPLA from E coli R515 (Re) TLRpureTM Sterile Solution
IAX-100-004 Lipid A from E. coli R515 (Re) TLRpureTM Sterlie Solution
IAX-100-006 LPS from E. coli O8:K27 (S-form) TLRpureTM Sterile Solution
IAX-100-007 LPS from E. coli R515 (Re) TLRpureTM TLRpureTM Sterile Solution
IAX-100-008 LPS from S. minnesota R595 (Re) TLRpureTM Sterile Solution
IAX-100-009 LPS (Universal) from S. abortus equi (S-form) TLRpureTM Sterile Solution
IAX-100-009B LPS from S. abortus equi (S-form) Biotin TLRpureTM Sterile Solution
IAX-100-010 LPS from E. coli EH100 (Ra) TLRpureTM TLRpureTM Sterile Solution
IAX-100-011 LPS from S. typhimurium (S-form) TLRpureTM Sterile Solution
IAX-100-012 LPS from E. coli O111:B4 (S-form) TLRpureTM Sterile Solution
IAX-100-013 LPS from E. coli O55:B5 (S-form) TLRpureTM Sterile Solution
IAX-100-014 LPS from E. coli J5 (Rc) TLRpureTM Sterile Solution
IAX-100-015 LPS from S. minnesota R345 (Rb) TLRpureTM Sterile Solution
IAX-100-016 LPS from S. minnesota R60 (Ra) TLRpureTM Sterile Solution
IAX-100-017 LPS from S. minnesota R5 (Rc) TLRpureTM Sterile Solution
IAX-100-018 LPS from S. minnesota R7 (Rd1) TLRpureTM Sterile Solution
IAX-100-019 LPS from S. enteritidis (S-form) TLRpureTM Sterile Solution
IAX-100-020 LPS from S. minnesota (S-form) TLRpureTM Sterile Solution
IAX-100-021 LPS from S. minnesota R3 (Rd2) TLRpureTM Sterile Solution
IAX-200-001 ODN 1668 (Type B) Endotoxin-free (sterile)
IAX-200-002 ODN 1826 (Type B) Endotoxin-free (sterile)
IAX-200-003 ODN 1585 (Type A) Endotoxin-free (sterile)
IAX-200-004 ODN M362 (Type C) Endotoxin-free (sterile)
IAX-200-005 ODN 2216 (Type A) Endotoxin-free (sterile)
IAX-200-006 ODN 2006 (Type B) Endotoxin-free (sterile)
IAX-200-007 ODN 2395 (Type C) Endotoxin-free (sterile)
IAX-200-050 iODN 2088 (Class I) Endotoxin-free (sterile)
IAX-200-051 iODN (ttaggg)4 (Class II) Endotoxin-free (sterile)
IAX-200-052 G-type iODN (Class I) Endotoxin-free (sterile)
IAX-200-053 Mini-iODN (Class I) Endotoxin-free (sterile)
IAX-200-054 Mega-iODN (Class I/ll) Endotoxin-free (sterile)
IAX-200-055 Duo-iODN (Class I) Endotoxin-free (sterile)
IAX-200-056 TLR7-iODN (inhibitory ODN) Endotoxin-free (sterile)
IAX-200-101 Trehalose 6,6’-dimycolate [Cord Factor] Endotoxin-free (sterile)
IAX-200-200 ODN 1720 (Control for ODN 1668) Endotoxin-free (sterile)
IAX-200-201 ODN 2138 (Control for ODN 1826) Endotoxin-free (sterile)
IAX-200-202 Neutral-ODN (Control for iODNs) Endotoxin-free (sterile)
IAX-200-203 ODN 2118 (Control for ODN 1585) Endotoxin-free (sterile)
IAX-200-204 ODN M383 (Control for ODN M362) Endotoxin-free (sterile)
IAX-200-205 ODN 2243 (Control for ODN 2216) Endotoxin-free (sterile)
IAX-200-206 ODN 2137 (Control for ODN 2006) Endotoxin-free (sterile)
IAX-200-208 CTRL2-ODN (Control for iODN and CpG-ODNs) Endotoxin-free (sterile)
IAX-400-002 Anti-TLR4 (Human) mAb (Blocking)
IAX-400-003 Anti-TLR4 (Human) mAb (2EII)
IAX-400-004 Anti-TLR4 (human) mAb (28H7)
IAX-600-001 IAXO-101
IAX-600-002 IAXO-102
IAX-600-003 IAXO-103
IAX-600-004 IAXO-201 (Control for IAXO-102)
IAX-600-005 IAXO-202 (Control for IAXO-101 and IAXO-103)
IAX-700-001 Argania Spinosa Kernel Oil (natural) (pure)
IAX-700-100 Lipodisq Control (sterile)
IAX-700-101 Nano-Curcumin Lipodisq (sterile)
IAX-700-102 Melatonin Lipodisq (sterile)
IAX-700-103 Metformin Lipodisq (sterile)
IAX-700-104 Oxyresveratrol Lipodisq (sterile)
IAX-700-105 Resveratrol Lipodisq (sterile)
IAX-700-106 Umifenovir Lipodisq (sterile)
IAX-700-107 Dexamethasone Lipodisq (sterile)
IAX-700-108 Ambroxol Lipodisq (sterile)
IAX-900-001 PBS Endotoxin-free (sterile)
IAX-900-002 ddWater Endotoxin-free (sterile)
IAX-900-003 Physiological Saline [NaCl 0.9%] Endotoxin-free (sterile)
IAX-900-004 PBS with EDTA Endotoxin-free (sterile)
IAX-900-005 TRIS with EDTA (TE Buffer) (100x)
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