Sequence

5’-gctcctagtagagatctcag-3’ (lower case letters: phosphorothioate linkage: nuclease resistant)

Synonyms

CTRL ODN-2, Control for inhibitory ODNs (iODNs) and CpG ODNs (TLR9 agonists)

MW

7,324 g/mol

Formulation

Lyophilised. Sterile. 100µg size includes 1.5ml ddWater Endotoxin-free (sterile) (Cat. No.: IAX-900-002-LD15). 1mg size includes 10ml ddWater Endotoxin-free (sterile) (Cat. No.: IAX-900-002-L010).

Endotoxin-free

Bacterial Endotoxin Test (kinetic turbidimetric LAL method) according to Ph. Eur. 9.

Passed according to specification:
Endotoxin-free: <0.002 EU/µg.

Sterility

Filter method: according to Ph. Eur. 9.

Passed according to specification:

• No growth in Thioglycolate medium at 30-35°C after 14 days.

• No growth in Soybean Casein Digest Broth (TSB) at 20-25°C after 14 days.

Handling

Keep sterile. Reconstitution: Dissolve total vial content in sterile endotoxin-free water or PBS. Add 50% of solvent and let dissolve for 10min. Add remaining 50% of the solvent and mix thoroughly. Moderate warming may aid dissolving.

Activity

Negative control ODN for in vivo use in rodents (50-150µg per injection).

As active use CpG ODN or iODN.